Modifying Enzymes
Supply Solutions Roche - RNase A, 100 mg; from bovine pancreas; pkg of 100 mg (10109169001) pkg of 25 mg (10109142001)
Roche - RNase A, 100 mg; from bovine pancreas; pkg of 100 mg (10109169001) pkg of 25 mg (10109142001)
Supply Solutions Roche - DNase I recombinant, RNase-free solution; from bovine pancreas, expressed in Pichia pastoris; pkg of 10,000 U; Hazardous: Y
Roche - DNase I recombinant, RNase-free solution; from bovine pancreas, expressed in Pichia pastoris; pkg of 10,000 U; Hazardous: Y
Supply Solutions Sigma Aldrich - Liberase Th Research Grade 100 Mg * Third Party Item
Sigma Aldrich - Liberase Th Research Grade 100 Mg * Third Party Item
Enzyme Research Laboratories Inc HUMAN KALLIKREIN 1 MG VIAL
Activation of Prekallikrein with Factor alpha -XIIa produces the enzymatically active Kallikrein. Kallikrein is a serine protease which consists of a heavy chain (Mr 52kD) and light chains (Mr either 36 or 33 kD) linked by disulfide bridges.
Rockland Immunochemicals BETA GALACTOSIDASE UNCONJUGATED - 0000-17
Beta Galactosidase is used as a control in beta-galactosidase based immunological assays.
New England Biolabs, Inc. Exonuclease VII – 200 units
Exonuclease VII, (Exo VII) derived from E. coli, cleaves single-stranded DNA (ssDNA) from both 5' to 3' and 3' to 5' direction. This enzyme is not active on linear or circular dsDNA. It is useful for removal of single stranded oligonucleotide primers from a completed PCR reaction when different primers are required for subsequent PCR reactions. Digestion of ssDNA by Exonuclease VII is metal-independent.
Supply Solutions Roche - b-Glucuronidase/Arylsulfatase; from Helix pomatia; pkg of 10 mL (10127698001) pkg of 2 mL (10127060001)
Roche - b-Glucuronidase/Arylsulfatase; from Helix pomatia; pkg of 10 mL (10127698001) pkg of 2 mL (10127060001)
Supply Solutions Roche - Proteinase K,Recomb.,Pcr Grd. Lyo.,25 Mg
Roche - Proteinase K,recomb.,PCR Grd. lyo.,25 mg; Lyophilized from Pichia pastoris; pkg of 100 mg (03115879001) pkg of 25 mg (03115836001) pkg of 2 x 250 mg (03115801001) pkg of 4 x 250 mg (0311582001)
Supply Solutions Roche - Collagenase A; from Clostridium histolyticum; pkg of 100 mg (10103578001) pkg of 2.5 g (11088793001) pkg of 500 mg (10103586001)
Roche - Collagenase A; from Clostridium histolyticum; pkg of 100 mg (10103578001) pkg of 2.5 g (11088793001) pkg of 500 mg (10103586001)
Supply Solutions Liberase™ DH Research Grade, high Dispase concentration, 2x5mg
The most commonly used method for decellularization procedures, such as tissue dissociation and cell harvesting, is based on the use of proteolytic enzymes. Proteases disrupt the extracellular matrix of tissue to allow the release of individual cells or the harvesting of cultured cells for transfer. The goal of cell isolation is to maximize the yield of dissociated cells that are viable and functionally active.
New England Biolabs, Inc. EnGen Sau Cas9 – 400 pmol
EnGen Sau Cas9 from Staphylococcus aureus is an RNA-guided DNA endonuclease that catalyzes site-specific cleavage of double-stranded DNA. Targeting requires a ~100 nucleotide guide RNA (gRNA) complementary to the target site, as well as a 5' NNGRRT 3 (N = any, R = A or G) protospacer adjacent motif (PAM) on the DNA strand opposite the target sequence. DNA cleavage by EnGen Sau Cas9 occurs ~3 bases 5' of the PAM. EnGen Sau Cas9 has Simian virus 40 (SV40) T antigen nuclear localization signals (NLS) at both the N- and C-termini of the protein. Schematic representation of Sau Cas9 nuclease sequence recognition and DNA cleavage
STEMCELL Technologies Hyaluronidase, Size: 50000 U
Hyaluronidase is a polysaccharidase consisting of a single polypeptide chain of 450 amino acid residues and contains four disulfide bonds. Hyaluronidase is glycosylated and contains 5% mannose and 2.17% glucosamine (Borders & Raftery). Hyaluronidase cleaves endo-N-acetlyhexosaminic bonds in hyaluronic acid and chondroitin sulfate A and C to tetrasaccharide residues, as hyaluronic acid and chondroitin sulfate are often found in connective tissues. Hyaluronidase is frequently used with other proteases such as collagenase.
Worthington Biochemical Corporation Deoxyribonuclease I, 25mg
Partially purified. A lyophilized powder. Store at 2-8°C. Bovine pancreatic deoxyribonuclease is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3' position and phosphate group at the 5' position. The average chain length of a limit digest is a tetranucleotide.
Worthington Biochemical Corporation CATALASE 10ML
Catalase, Filtered, Minimum Activity: 40000U/mg Protein, Supplied as an aqueous solution of 2X crystallized catalase filtered through a 0.22u pore size Membrane, Store at 2 deg. to 8 deg.C, 10mL
Discovery Life Sciences HUM CYP2C8+REDUCTASE+B5 0.5NMO
Human CYP2C8 + P450 Reductase + Cytochrome b5 SUPERSOMES, 0.5 nmole cytochrome P450 in 0.5 ml. Store at -80degreeC Catalytic assay: paclitaxel-6(alpha)-hydroxylase activity, representative catalytic activity: 2,200 pmole/(mg x min)